Data Availability StatementThe data used to aid the findings of this study are available from the corresponding author upon request. inhibitory effects on osteosarcoma and different effects on osteoblasts. And, with the increase of the content of norcantharidin, the antitumor performance of the composite has been enhanced. In summary, the nano-SHAP system developed in this study is a drug delivery material that can inhibit the growth of tumors and induce the proliferation of osteoblasts. 1. Introduction Osteosarcoma (OS) is the most common primary malignant solid tumor in humans between the ages of 10 and 25 [1]. It is characterized mainly by the destruction of and recurrence in local tissue and is prone to distant metastasis. The widely accepted treatment for OS, which includes routine surgical resection and neoadjuvant chemotherapy, can improve the short-term survival rate to 60?70% [2]. However, tumor cells can remain in the area around the tumor resection site after surgery, resulting in high bone tissue and recurrence destruction. Adjuvant chemotherapy has a significant function in getting rid of the rest of the tumor cells and preventing tumor recurrence and metastasis [3]. In addition, it’s quite common understanding that bone tissue defects have to be fixed using fix techniques such as for example allogeneic bone tissue transplantation, iliac bone tissue grafts, and irradiation bone tissue grafts. However, you may still find drawbacks in these technology. For example, allogeneic bone is one of the most commonly used materials utilized for fixing tumor-induced bone defects, but it has no antitumor effect and is associated with the spread of infectious diseases and immune response [4]. Therefore, biocompatible biomaterials have the potential to be anticancer agents and to repair defects. Composite materials, which comprise two or more excellent biomaterials, exhibit the properties of those materials. In recent years, composite materials composed of inorganic and organic components have drawn increasing attention. Depending on the characteristics of the components, the composites are used in catalysts, genes, and drug service providers [5, 6], for photodynamic and photothermal effects [7], and for their antibacterial properties [8, 9]. The organic components are important for modifying the inorganic TSA pontent inhibitor materials, improving biocompatibility, escaping mononuclear cell phagocytic systems, and improving the therapeutic TSA pontent inhibitor effects of the composite [10]. The cited studies have shown that composite materials will have a wide range of applications in the biomedical field and in many different scientific fields in the future. Inorganic salts and carbohydrates play a crucial role in antitumor, bone regeneration, and biomineralization. Chitosan (CS) is the only basic aminopolysaccharide found in natural polysaccharides. It is widely used in many fields because of its biocompatible, biodegradable, and good antibacterial and antioxidant properties. CS can be used to absorb metal ions or other chemicals which is used to resist tumors [11]. Nanohydroxyapatite (nHAP) is the primary inorganic element of individual bones and tooth, accounting for 50C70% of individual bone tissue. Because of its great biocompatibility, natural activity, and osteoinductivity, nHAP put into the CS materials can increase mechanised functionality and imitate the natural framework of bone tissue while preserving biocompatibility [12]. Furthermore, studies show that CS/nHAP components increase bone tissue regeneration mainly by upregulating osteogenic genes and marketing mesenchymal stem cell (MSC) differentiation mineralization in vivo [13, 14]. Because of the advantages of great stability, biocompatibility, bone ENAH tissue conductivity, and bone tissue induction, CS/nHAP components have been created as TSA pontent inhibitor osteogenic components. Strontium (Sr) ions not merely promote the forming of brand-new bone tissue but also inhibit bone tissue resorption [15], that have results on marketing osteogenic differentiation of MSCs, avoiding the proliferation of osteoclasts [16], and raising the osteoinductivity from the amalgamated. Marie et al. [17] reported that low-dose Sr2+ may accelerate bone tissue boost and formation bone tissue mass TSA pontent inhibitor in pets. In the scholarly research by Lei et al. [15], SrHAP/CS was proven to have excellent cell compatibility and that it supports adhesion, diffusion, and proliferation of human bone marrow stromal cells (hBMSCs). Sr2+ released by the material contributed to osteogenic differentiation, and the presence of Sr in the SrHAP/CS material enhanced alkaline phosphatase (ALP) activity, extracellular matrix (ECM) mineralization, and the expression of osteogenesis-related genes Col-1 and ALP. Furthermore, the percentage of Sr in SrHAP experienced a large effect on ALP activity. ALP activity gradually increased as the Sr/Ca ratio in SrHAP increased and reached the maximum when the Sr/Ca ratio was 1?:?1. A high dose of Sr may lead to bone mineralization defects and even cause bone abnormalities. Norcantharidin.